Part:BBa_K3814004
free-use GFP (fuGFP)
fuGFP, short for ‘free-use GFP’, is an open source GFP developed by Mark Somerville and Nick Coleman at the University of Sydney. The rationale for developing this GFP variant was to make a superfolding GFP (sfGFP) that was not protected by patents (unlike the original sfGFP), thus facilitating all kinds of synbio research, whether or not this has commercial intent. The mechanism for moving the GFP off-patent was to use DNA shuffling to derive a synthetic gene encoding a protein with <80% amino acid identity to sfGFP, since the patent claims everything at >80% identity. fuGFP preferentially absorbs long-wave UV light and emits green. Note that this is different to sfGFP, which has an S65T mutation that makes it absorb more blue light. fuGFP is available as pUS252 in AddGene.
Figure 1. Expression of fuGFP in E.coli strain TOP10.
References
Coleman, N., & Somerville, M. (2019, May). The Story of Free Use GFP (fuGFP). Small Things Considered. https://schaechter.asmblog.org/schaechter/2019/05/the-story-of-free-use-gfp-fugfp.html
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 151
- 1000COMPATIBLE WITH RFC[1000]
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