Part:BBa_K259000

fhuA - Outer membrane transporter for ferrichrome-iron

Biology of fhuA

fhuA is an outer membrane (OM) protein that is found in the gram negative bacterium, E.coli. It is involved in the active-transport (energy-dependent uptake) of ferrichrome-iron from the extracellular environment. However fhuA is only able to bind ferrichrome-iron (i.e. ferrichrome iron is a ligand of fhuA protein). For the uptake to be completed fhuA needs to form a complex with another protein, tonB that carries out the energy-dependent uptake by using ATP as substrate to undergo the relevant conformational changes.

What this Biobrick gives you upon expression

This biobrick encodes ONLY for fhuA.

fhuA Statistics

fhuA is a beta-barell protein (22 anti-parallel beta-strands) and is composed of 747 aminoacids. This receptor protein may be used as an attachment point by some colicins.

Chassis	Monomer/Multimer	Aminoacid Length/Weight	Localisation	Structure
E.Coli	Monomer	747/82.182kDa	Outer Membrane	beta-barrel

References

Locher K.P., Rees B., Koebnik R., Mitschler A., Moulinier L., Rosenusch J.P. and Moras D. (1998),Transmembrane signalling across the Ligand-Gated FhuAReceptor: Crystal structures of Free and Ferrichrome-Bound States reveal allosteric changes. Cell 95,771-778.

[http://www.uniprot.org/uniprot/P06971 Uniprot access number P06971]

[http://ecogene.org/geneInfo.php?eg_id=EG10302 EcoGene Access Number EG10302]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 682
  Illegal AgeI site found at 820
  Illegal AgeI site found at 1225
  Illegal AgeI site found at 1999
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 724