Part:BBa_K4421003
scfv library with 10 individual colonies
This part is a phage display scfv library which consists of ten kinds of individual colonies.The sequence encoding the scFv antibodies generated from cetuximab,trastuzumab, CH65, 9.8B, 2F5, F10, 7D11, 8D6, omalizumab, TE33, R10 and HC33.8 was chemically synthesized. The sequence information can be found in the Protein Data Bank. And we next aim to construct a CAR-NK-92 cell library of 10 kinds of unique CAR constructs with this part.
Usage and Biology
Antibody libraries can be constructed based on different sources, such as immunized animals or naturally immunized or infected humans, or naive immune systems, which can be derived from nonimmune natural or computational and synthetic sources.In vitro experiments, the MCF-7 cells and the derivative cells were characterized, which have been used in our previous study.A small library of 10 CAR constructs comprising antigen-specific scFv(BBa K4421003) were constructed, termed as the NK-92CTX-TTZ library.And the sequence encoding the scFv antibodies was generated from cetuximab(CTX), trastuzumab(TTZ), CH65, 9.8B, 2F5, F10, 7D11, 8D6, omalizumab, TE33, R10 and HC33.8.
Background and detail description
Used for a CAR
In. our project,
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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