![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4011996
Designed by: Sha Zhou Group: iGEM21_LINKS_China (2022-03-29)
protein
abcabcabcabc
Usage and Biology
sfkerrl
Design Considerations
cmgnsofgd
Characterization
tsodvndkg
![](/wiki/images/a/a6/T--LINKS_China--Figure_16.png)
Figure 1: Construction and expression of TnaA-FMO proteins. A) Schematic representing TnaA-RBS-FMO, TnaA-Flexible linker-FMO, and TnaA-Rigid linker-FMO transformed into E. coli DH5a ΔTnaA. B. Schematic representing the structure of TnaA-linker-FMO. C) The sequences between TnaA and FMO, with the rbs and amino acids labelled. FL and RL stands for flexible linker and rigid linker respectively. D) SDS-PAGE analysis of TnaA-RBS-FMO, TnaA-RL-FMO, and TnaA-FL-FMO.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2812
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1219
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |