Coding

Part:BBa_K4011996

Designed by: Sha Zhou   Group: iGEM21_LINKS_China   (2022-03-29)
Revision as of 07:37, 29 March 2022 by LeviZ (Talk | contribs)

protein

abcabcabcabc

Usage and Biology

sfkerrl

Design Considerations

cmgnsofgd

Characterization

tsodvndkg

Figure 1: Construction and expression of TnaA-FMO proteins. A) Schematic representing TnaA-RBS-FMO, TnaA-Flexible linker-FMO, and TnaA-Rigid linker-FMO transformed into E. coli DH5a ΔTnaA. B. Schematic representing the structure of TnaA-linker-FMO. C) The sequences between TnaA and FMO, with the rbs and amino acids labelled. FL and RL stands for flexible linker and rigid linker respectively. D) SDS-PAGE analysis of TnaA-RBS-FMO, TnaA-RL-FMO, and TnaA-FL-FMO.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2812
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1219
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None