Coding

Part:BBa_K194002

Designed by: Christian Kaas   Group: iGEM09_DTU_Denmark   (2009-07-13)
Revision as of 21:04, 13 July 2009 by Registry (Talk | contribs)

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Destabilized GFP for yeast

This variant of biobrick BBa_K194001 has been destabilized by fusing biobrick BBa_K194000. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


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Categories
Parameters
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