Regulatory

Part:BBa_K116401

Designed by: Chih-Hsien Yang   Group: iGEM08_NYMU-Taipei   (2008-10-29)
Revision as of 19:23, 17 December 2021 by Manasvigupta (Talk | contribs) (→‎Experience)


external phosphate sensing promoter

Promoter of phoB in E.coli, an external phosphate regulated promoter that can be activated when encountering phosphate limitation.


Experience

Utilizing the modified phosphate range and characterization protocol (See: Modified Protocol), we characterized the BBa_K2447000 phosphate sensor. The characterization curve (Figure 1) showed a linear, negative trend throughout phosphate concentrations ranging from 0-100μM. This data closely paralleled the predictive ODE model created by our 2020 team and the previous characterization data by NUS Singapore iGEM 2017.

Figure 1. Characterization curve for BBa_K2447000 for phosphate concentrations between 0μM and 100μM.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
Parameters
negative_regulators
positive_regulators1