Composite
Pre B2

Part:BBa_K4060105

Designed by: YU CHUAN LEE   Group: iGEM21_NYCU-Taipei   (2021-09-30)
Revision as of 14:28, 7 December 2021 by JasonCTS (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


-- No description --

BBa_K4060101 with thermometer RBS

The gene fragment Pre B2 is composed of Pre A2 and the Thermometer RBS BBa_K115002. We digested the vector fragment BBa_K115002 with EcoRI and XbaI and the insert fragment Pre A2 with EcoRI and SpeI. We ligate these fragments together to form Pre B2.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None