Part:BBa_K4059054:Design
CPR from Phanerochaete chrysosporium with N-terminal substitution (14), E. coli K12 codon optimized,
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 1483 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene encodes a NADPH-cytochrome P450 reductase (CPR) delivers the electrons necessary for the reaction carried out by the 4-monooxygenase (PsiH). This gene originates from the mushroom Phanerochaete chrysosporium. CPR is like PsiH usually bound to the membrane of the endoplasmatic reticulum by the N-terminal, which is strongly hydrophobic. When expressed in bacteria that lack the endoplasmatic reticulum, the N-terminal can affect the folding of the enzyme leading to an ineffective protein. Therefore N-terminal modifications are needed. This brick includes a modification in form of a substitution of an entire N-terminal from another enzyme, which is soluble within the cell.
Source
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