Composite
Pre B3

Part:BBa_K4060106

Designed by: YU CHUAN LEE   Group: iGEM21_NYCU-Taipei   (2021-09-30)
Revision as of 03:44, 22 October 2021 by JasonCTS (Talk | contribs) (→‎Usage and Biology)


Pre B3 gene

a kill switch strategy.

Usage and Biology

The RFP expression of Construct 3 We used RFP(E1010) to monitor the expression of TetR, so that we can figure out how L-arabinose will influence the expression of TetR in our Design 1.

<img src="https://2021.igem.org/File:T--NYCU-Taipei--kilksw_result_1.png" alt="">

<img src="https://2021.igem.org/File:T--NYCU-Taipei--kilksw_result_2.png" alt="">

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 711
    Illegal AgeI site found at 823
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None