Composite

Part:BBa_K3738015:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)
Revision as of 03:40, 22 October 2021 by Marklea (Talk | contribs) (Source)


MS2 Bacteriophage Capsid Protein with N-Terminal Histidine Tag (Composite Part)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 485
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 485
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 485
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 485
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The MS2 capsid protein coding region in this part has been codon-optimized for E.coli.


Source

Ms2 Bacteriophage

References