Part:BBa_K3844003:Sequence, Features, and Subparts
1. Function
This is a transcription factor that binds to the Styrene VOC. It mainly regulates and represses the transcription of certain genes.
2. Design Consideration
Our project requires for the transcription factor to be dependent on the presence of VOCs. When researching the design of trimethylbenzene, the VOC that this transcription factor binds to, we needed to make sure that, hypothetically, everything would work based on our model. After checking through extensive research that our design would fit with this transcription factor, we chose it.
3. Chracterization
atggaagtgaacgcgggcggcgtgattgcgtatattagcagcagcagcagcgcgagcagc ccggcgagctgccatagcgaaggcagcgaaaacagctttcagagcagcagcagcagcgtg ccgagcagcccgaacagcagcaacagcgataccaacggcaacccgaaaaacggcgatctg gcgaacattgaaggcattctgaaaaacgatcgcattgattgcagcatgaaaaccagcaaa agcagcgcgccgggcatgaccaaaagccatagcggcgtgaccaaatttagcggcatggtg ctgctgtgcaaagtgtgcggcgatgtggcgagcggctttcattatggcgtgcatgcgtgc gaaggctgcaaaggcttttttcgccgcagcattcagcagaacattcagtataaaaaatgc ctgaaaaacgaaaactgcagcattatgcgcatgaaccgcaaccgctgccagcagtgccgc tttaaaaaatgcctgagcgtgggcatgagccgcgatgcggtgcgctttggccgcattccg aaacgcgaaaaacagcgcatgctgattgaaatgcagagcgcgatgaaaaccatgatgaac agccagtttagcggccatctgcagaacgataccctggtggaacatcatgaacagaccgcg ctgccggcgcaggaacagctgcgcccgaaaccgcagctggaacaggaaaacattaaaagc agcagcccgccgagcagcgattttgcgaaagaagaagtgattggcatggtgacccgcgcg cataaagatacctttatgtataaccaggaacagcaggaaaacagcgcggaaagcatgcag ccgcagcgcggcgaacgcattccgaaaaacatggaacagtataacctgaaccatgatcat tgcggcaacggcctgagcagccattttccgtgcagcgaaagccagcagcatctgaacggc cagtttaaaggccgcaacattatgcattatccgaacggccatgcgatttgcattgcgaac ggccattgcatgaactttagcaacgcgtatacccagcgcgtgtgcgatcgcgtgccgatt gatggctttagccagaacgaaaacaaaaacagctatctgtgcaacaccggcggccgcatg catctggtgtgcccgctgagcaaaagcccgtatgtggatccgcataaaagcggccatgaa atttgggaagaatttagcatgagctttaccccggcggtgaaagaagtggtggaatttgcg aaacgcattccgggctttcgcgatctgagccagcatgatcaggtgaacctgctgaaagcg ggcacctttgaagtgctgatggtgcgctttgcgagcctgtttgatgcgaaagaacgcacc gtgacctttctgagcggcaaaaaatatagcgtggatgatctgcatagcatgggcgcgggc gatctgctgaacagcatgtttgaatttagcgaaaaactgaacgcgctgcagctgagcgat gaagaaatgagcctgtttaccgcggtggtgctggtgagcgcggatcgcagcggcattgaa aacgtgaacagcgtggaagcgctgcaggaaaccctgattcgcgcgctgcgcaccctgatt atgaaaaaccatccgaacgaagcgagcatttttaccaaactgctgctgaaactgccggat Ctgcgcagcctgaacaacatgcatagcgaagaactgctggcgtttaaagtgcatccg