Part:BBa_K3745043
Plac+rhlABC
The plasmid contains Plac, rhlA, rhlB, and rhlC. Therefore, the plasmid plays a significant role in the synthesis of rhamnolipids in E. coli. With the help of this plasmid and espacially rhlA, B, and C, three major materials can compound to form rhamnolipids: Glycolysis or Gluconeogenesis, ß-oxidation, and Fatty acid. To be specific, 3-hydroxyacyl-CoA that is derived from ß-oxidation can compound with 3-hydroxyacyl-ACP under a special enzyme——RhlA to form 3-hydroxyalanoyl-3hydroxyalkanoyl(HAA)-ACP or S-CoA. Eventually, dTDP-L-rhamnose and HAA will combine together under the catalytic effects of RhlB to forge Mono-rhamnolipids. And then, with the assistance of RhlC, Mono-rhamnolipids can be turned into Di-rhamnolipids which are experimentally proved to be a better biosurfactant than mono-rhamnolipid.
It is worth informing that the Plac in the DNA feature's graph is a reverse promoter (activate LacI instead of activating rhlA, B, and C). The arrow on the feature is not accurate due to the reason that the image of the promoter won't appear in the reverse direction. Nevertheless, the DNA sequence is posted in the correct arrangement which Plac is responsible for activating LacI.
Figure 1.Result of HPLC-MS measurement of IPTG induced culture
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 7
Illegal NotI site found at 3546
Illegal NotI site found at 4759 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2171
Illegal NgoMIV site found at 2480
Illegal NgoMIV site found at 4089
Illegal AgeI site found at 3092 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 4228
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