Coding

Part:BBa_K3945002

Designed by: Ramin Kahidi   Group: iGEM21_Calgary   (2021-10-20)
Revision as of 03:30, 22 October 2021 by ArshiaMostoufi (Talk | contribs)

FC-Mutated Lanmodulin (FC LanM)


Usage and Biology

Lanmodulin is recently a novel lanthanide binding protein from Methylobacterium extorquens that displays more than 100-million fold selectivity for rare earth elements [1]. Such affinity and selectivity have not been observed in any previously studied macromolecule. In addition, lanmodulin is extremely robust, capable of withstanding temperatures as high as 95 °C and pH levels as low as 2.5 [2]. Thus, allowing it to be the perfect molecular tool for us in an efficient rare earth recovery system.

We have mutated the lanmodulin sequence using Family Conservation Algorithm to improve the binding capacity of lanmodulin's fourth binding pocket.

Design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 111
    Illegal BamHI site found at 186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

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Categories
Parameters
None