Composite

Part:BBa_K3738010:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)
Revision as of 03:30, 22 October 2021 by Marklea (Talk | contribs) (→‎References)

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MS2 Bacteriophage Capsid Protein with Maturation Protein A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1490
    Illegal NheI site found at 1513
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal XhoI site found at 1075
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1609
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding regions of this part have been codon-optimized for use in E.coli.


Source

MS2 Bacteriophage

References

1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664
[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138 https://www.uniprot.org/uniprot/P03612