Composite

Part:BBa_K3738009:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)
Revision as of 03:28, 22 October 2021 by Marklea (Talk | contribs) (→‎References)


MlrA (Microcystinase) Composite Part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 556
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The microcystinase coding region in this part has been codon-optimized for E.coli.


Source

Sphingomonas sp. USTB-05

References

Wei J, Huang F, Feng H, Massey IY, Clara T, Long D, et al. Characterization and Mechanism of Linearized-Microcystinase Involved in Bacterial Degradation of Microcystins. Frontiers in Microbiology. 2021;12(527). doi: 10.3389/fmicb.2021.646084.