Tag

Part:BBa_K4031015

Designed by: Yuichiro Ikagawa   Group: iGEM21_Gifu   (2021-10-21)
Revision as of 03:26, 22 October 2021 by Ikachan (Talk | contribs)

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Maltose binding protein (MBP) tag

MBP is a hydrophilic tag that can be fused to a protein of interest to increase its expression level and solubility. MBP tag also promotes proper folding of the fusion protein. MBP fusion proteins expressed in plasmid vectors carrying the MBP domain can be purified in a single step by affinity chromatography using amylose resin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None