Coding

Part:BBa_K3738008:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)
Revision as of 03:24, 22 October 2021 by Emily Hagens (Talk | contribs) (→‎References)


MS2 Bacteriophage Capsid Protein Dimer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 138
    Illegal PstI site found at 546
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 138
    Illegal PstI site found at 546
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 138
    Illegal PstI site found at 546
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 138
    Illegal PstI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal histidine tag and has had the polyarginine tag removed.

This part has not yet been codon-optimized.

Source

This part comes from the MS2 bacteriophage.

References

de Martín Garrido, N., Crone, M.A., Ramlaul, K., Simpson, P.A., Freemont, P.S. and Aylett, C.H.S. (2020), Bacteriophage MS2 displays unreported capsid variability assembling T = 4 and mixed capsids. Mol Microbiol, 113: 143-152. https://doi.org/10.1111/mmi.14406

[1] Glasgow, J., Capehart, S., Francis, M., and Tullman-Ercek, D. (2012) Osmolyte-Mediated Encapsulation of Proteins inside MS2 viral capsids. ACSNAno. 6, 8658-8664

[2] Vives, E., Schmidt, J., And Pelegrin, A. (2008) Cell penetrating and cell-targeting peptides in drug delivery. Biochimica et biophysica Acta. 1786, 126-138

https://www.uniprot.org/uniprot/P03612