Coding

Part:BBa_K3738003:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)
Revision as of 02:40, 22 October 2021 by Emily Hagens (Talk | contribs) (→‎References)

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MS2 Bacteriophage Maturation Protein A with N-Terminal Histidine Tag and TEV Cleavage Site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 601
    Illegal BamHI site found at 875
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 964


Design Notes

This part is a modified version of BBa_K3738000, to which an N-terminal histidine tag and TEV cleavage site have been added. As maturation protein A has not been well studied for use in phage-like particles, we have designed various versions of the part for testing purposes.

This part has been codon optimized for use in E.coli.

Source

This part comes from the MS2 Bacteriophage.

References

https://www.uniprot.org/uniprot/P03610

Meng, R., Jiang, M., Cui, Z. et al. Structural basis for the adsorption of a single-stranded RNA bacteriophage. Nat Commun 10, 3130 (2019). https://doi.org/10.1038/s41467-019-11126-8