RNA
Part:BBa_K3714010
Designed by: Bairun Chen Group: iGEM21_SJTU-BioX-Shanghai (2021-09-30)
Improved gard-337 for lateral flow assay
To test our optimized design with TMSD(toehold mediated strand displacement) reaction, a toehold site between biotin probe binding site and aptazyme domain is necessary. However, designing the probe manually and testing with available RNA structure prediction model is a huge work with consuming much labor.
This part was designed by our probe design model and verified by the prediction model of team: SJTU-Software. And the changed sequences shows no influence on the function of aptazyme, the toehold worked as our expect as well (Figure.1).
The stop oligo significantly in
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//rna/aptazyme
None |