RNA

Part:BBa_K3714010

Designed by: Bairun Chen   Group: iGEM21_SJTU-BioX-Shanghai   (2021-09-30)
Revision as of 02:24, 22 October 2021 by Grus (Talk | contribs)


Improved gard-337 for lateral flow assay

To test our optimized design with TMSD(toehold mediated strand displacement) reaction, a toehold site between biotin probe binding site and aptazyme domain is necessary. However, designing the probe manually and testing with available RNA structure prediction model is a huge work with consuming much labor.
This part was designed by our probe design model and verified by the prediction model of team: SJTU-Software. And the changed sequences shows no influence on the function of aptazyme, the toehold worked as our expect as well (Figure.1).
The stop oligo significantly in

K3714016.png
Figure.1 | Verification of temporarily inhibition and disinhibition by oligonucleotides. The aptazyme was produced by a T7 in vitro transcription system. The product was run on a denatured PAGE. Gardiquimod is an inhibitor of the cleavage activity and "-gardiquimod" is a control group. "stop" and "start" are two oligonucleotides designed to control the activity of the aptazyme/

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
//rna/aptazyme
Parameters
None