RNA
Part:BBa_K3714010
Designed by: Bairun Chen Group: iGEM21_SJTU-BioX-Shanghai (2021-09-30)
Improved gard-337 for lateral flow assay
To test our optimized design with TMSD(toehold mediated strand displacement) reaction, a toehold site between biotin probe binding site and aptazyme domain is necessary. However, designing the probe manually and testing with available RNA structure prediction model is a huge work with consuming much labor. This part was designed by our probe design model and verified by the prediction model of team: SJTU-Software. And the changed sequences shows no influence on the function of aptazyme, the toehold worked as our expect as well (Figure.1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//rna/aptazyme
None |