Regulatory
dpsara

Part:BBa_K3790086

Designed by: Chongwen Cao   Group: iGEM21_Fudan   (2021-10-01)
Revision as of 02:05, 22 October 2021 by Chongwen (Talk | contribs)


pBAD+dps promoter fusion


Introduction

2021 Fudan

It's designed as an inducible σS promoter, but we didn't test it.

We wanted to use this part to prove our hypothesis that the simplest form of σS promoter can be combined with other regulatory part and function as expected.



Usage and Biology

An inducible σS promoter




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//promoter
Parameters
None