Composite

Part:BBa_K233001

Designed by: Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil   Group: iGEM09_IBB_Pune   (2009-07-10)
Revision as of 06:22, 10 July 2009 by Registry (Talk | contribs)

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Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway

this is a module that contains the following components 1.an inducible lac promoter with the operator( lacI binding sites) 2. RBS (ribosome binding site) 3. an export tag viz. torA 4. beta-galactosidase gene 5. a reporter gene viz. GFP 6. TERM

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4099


[edit]
Categories
Parameters
None