Coding

Part:BBa_K3739003

Designed by: Han Ziyan   Group: iGEM21_XMU-China   (2021-09-08)
Revision as of 01:41, 22 October 2021 by Junio (Talk | contribs) (→‎Characterization)


his-GFP

his-tag is used to be detected by anti-his antibody and purify the his-linking fusion, GFP can emit green fluorescence.

Biology

GFP Protein tags are peptides genetically fused to the proteins of interest. There are many different kinds of tags developed for for different purposes. For labeling experiments, normally the proteins of interest are tagged directly by fluorescent tags. Green fluorescent protein (GFP) make it easier to study protein localization, interactions and dynamics when fusing with other peptides and proteins.

Usage

Used to construct the expression system and obtained the composite part BBa_K3739043.

Characterization

1.Colony PCR We construct his-GFP CDS with J23100 promotor and B0030 RBS, the transformed cells are selected by colony PCR. The experiment result is showed in Fig. 1. alt text Fig. 1. DNA gel electrophoresis of colony PCR product of J23100-B0030-his-GFP-pSB1C3. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 73


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Categories
Parameters
None