Reporter

Part:BBa_K3945011:Design

Designed by: Thuy-Ann Tran   Group: iGEM21_Calgary   (2021-10-20)
Revision as of 00:34, 22 October 2021 by ArshiaMostoufi (Talk | contribs) (→‎Source)


Elektra 1: An electrochemical- measurement system for rare earth elements


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 89
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 89
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 89
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 89
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The reason histidine was chosen to be the amino acid that would covalently attach to the ruthenium molecule was because there were no other histidine amino acids in lanmodulin. This will allow for selective binding as it is very important that there are no other ruthenium molecules that are attached to lanM except on the end (as it could affect REE binding in addition to creating false positives in Elektra).

Source

References