Composite

Part:BBa_K4006012:Design

Designed by: Margaret Cook   Group: iGEM21_ASU   (2021-10-21)
Revision as of 00:34, 22 October 2021 by Mkcook (Talk | contribs) (→‎Source)

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MT-IEE5-arsC-IEE2-fTNa


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 606
    Illegal BamHI site found at 40
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 224
    Illegal SapI site found at 1709
    Illegal SapI.rc site found at 31
    Illegal SapI.rc site found at 2219


Design Notes

This part was designed for Golden Gate assembly and use with the type IIS restriction enzyme SapI in the compatible plasmid backbone pASapI. It is codon optimized for use in the chloroplast of Chlamydomonas reinhardtii.

Source

Part BBa_K3275000 - Human MT sequence
10.1007/s11103-018-0776-z - Chlamydomonas reinhardtii origin IEE2
Uniprot ID P08692 - E.coli ArsC sequence
10.1007/s11103-018-0776-z - Chlamydomonas reinhardtii origin IEE5
Uniprot FTNA_ECOLI P0A998 - E.coli FtnA sequence

References