Reporter

Part:BBa_K3945011

Designed by: Thuy-Ann Tran   Group: iGEM21_Calgary   (2021-10-20)
Revision as of 00:31, 22 October 2021 by ArshiaMostoufi (Talk | contribs) (Design)

Elektra 1: An electrochemical- measurement system for rare earth elements


Usage and Biology

The Elektra system is compromised of the lanmodulin protein with the addition of a couple of key amino acids on its terminal ends. The cysteine amino acid on the C-terminal end will be used to covalently couple lanmodulin to a gold electrode via EDC coupling. On the N-terminal end of lanmodulin, there is a histidine residue which will allow a ruthenium signal molecule to be attached onto the other end of lanmodulin to create Elektra. Upon binding to lanthanides the N-terminal end of lanmodulin will be brought closer to the gold electrode and at a certain voltage, the ruthenium molecule redox potential will change, emitting an electrochemical signal that can be measured using a potentiostat. As such Elektra can quickly transform protein metal binding to a measurable electrochemical signal.

Design

The reason histidine was chosen to be the amino acid that would covalently attach to the ruthenium molecule was because there were no other histidine amino acids in lanmodulin. This will allow for selective binding as it is very important that there are no other ruthenium molecules that are attached to lanM except on the end (as it could affect REE binding in addition to creating false positives in Elektra).

Experimental Workflow

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 89
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 89
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 89
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 89
  • 1000
    COMPATIBLE WITH RFC[1000]

References

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Categories
Parameters
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