Reporter

Part:BBa_K3871004:Design

Designed by: Yoav Navott   Group: iGEM21_TAU_Israel   (2021-10-21)
Revision as of 00:28, 22 October 2021 by Ynavott (Talk | contribs) (→‎Design Notes)


mCherry, TDR-D optimized for Bacillus subtilis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 364
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 364
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mCherry is a red fluorescent protein used as a reporter derived from the fluorophore DsRed, which wasthat originally isolated from Discosoma sea anemones. It is commonly used due to its colour and photostability compared to other monomeric fluorophores.

This part is a variant of mCherry, the sequence of which was modified to optimize its expression in Bacillus subtilis against E. coli using the Communique tool developed by the 2021 TAU Israel team. This specific variant was optimized based on typical decoding rate difference between B. subtilis and E. coli. You may read more about the optimization model here.


For more information on this part, please consult the 2021 TAU Israel wiki results page found here. Information about experimental procedures can be found here and here.

Source

mCherry is one of several "second-generation" monomeric fluorescent proteins developed in Roger Tsien's laboratory at UCSD (cf., Nature Biotechnology 22, 1567 - 1572 (2004). PMID 15558047

References