Regulatory

Part:BBa_K3893000

Designed by: Arly Armas   Group: iGEM21_Ecuador   (2021-10-20)
Revision as of 00:27, 22 October 2021 by Acarmas6 (Talk | contribs)


Shine Dalgarno for E coli

This sequence is part of the modular platform for producing dsRNAs in the proyect AgroBactory 593.

The Shine-Dalgarno (SD) is a sequence located 4 to 10 bases upstream of start codons and it is often 4 to 6 nucleotides long (most common: 5' AAGAG 3'). During translation, the SD sequence join to the 3' end of the 16S rRNA by base pairing with the anti-SD sequence (ASD); this mating helps the ribosomes to reach the right position of the start codon and therefore, translation can begin [1]. In synthetic biology, the SD sequence has been used to increase translation rate and as well as the yield of protein production [2]. On the other hand, for interference RNA (RNAi) assays, studies has shown that the SD sequence provides stability to the dsRNA molecules, preventing its degration [3]. For this reason, we decided to attach the SD sequence to our parts.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



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Categories
Parameters
None