Part:BBa_K3889150
SP200+SRTF1 Binding Site+ RBS
SP200 (BBa_K3889012) fused with SRTF1 binding site (BBa_K3889030) and RBS (BBa_K1351031) in order to create a progesterone sensitive promoter if SRTF1 (BBa_K3889021) is being expressed.
In order to produce P22 c2 repressor (BBa_K3889020) in synchronisation with progesterone this part was used as in BBa_K3889101.
Results
In this graph, SRTF1 cassette (BBa_K3889100) and P22 Cassette(BBa_K3889101) were co-expressed in Bacillus subtilis 168 consisting of SRTF1 with mCherry reporter and P22 Cassette with Azurite reporter under various concentrations of progesterone.
Unexpectedly, the mCherry expression decreased at higher concentrations of progesterone. Further experimental evidence is needed to determine the cause. Additionally, the Azurite expression increased with progesterone levels signifying that p22 protein production increased. Hence, there is evidence to suggest that SRTF1 and SRTF1 DNA binding sites were interacting as expected and progesterone sensing is taking place in Bacillus subtilis.
results Table:
| Progesterone Conc. (in nM) | Azurite | mCherry |
|---|---|---|
| 0 | 11374 | 297821 |
| 16.5 | 17101 | 281770 |
| 33 | 17275 | 287161 |
| 82.5 | 29714 | 249459 |
| 165 | 45536 | 153234 |
| 330 | 78069 | 117241 |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |