Coding

Part:BBa_K4088898

Designed by: Ruslan Vasiliev, Yaroslav Ugolkov, Alice Potapova, Pavel Ivanov-Rostovtsev   Group: iGEM21_Moscow_City   (2021-10-21)
Revision as of 00:04, 22 October 2021 by Registry (Talk | contribs)

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dCas13a

Cas13a is a classic RNA-targeting nuclease that is widely used in a variety of diagnostics methods. It is a very specific variant of RNA detection, it can be customized to recognize single nucleotides on the target RNA. dCas13a is a catalytically dead LwaCas13a enzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 622
    Illegal BglII site found at 924
    Illegal BglII site found at 1689
    Illegal BglII site found at 1956
    Illegal BglII site found at 2166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None