Coding

Part:BBa_K4088895

Designed by: Ruslan Vasiliev, Yaroslav Ugolkov, Alice Potapova, Pavel Ivanov-Rostovtsev   Group: iGEM21_Moscow_City   (2021-10-21)
Revision as of 23:54, 21 October 2021 by Registry (Talk | contribs)

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C-lact (int)

C-terminal beta-lactamase - half of the gene that encodes beta-lactamase (AmpR). It can fuse with N-terminal beta-lactamase to fully assemble beta-lactamase that has the ability to hydrolyze nitrocefine allows beta-lactamase to be used as a reporter protein where solution color is changing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 138


[edit]
Categories
Parameters
None