Part:BBa_K3792003

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BIOFAB apFAB40 Measurement Device

apFAB40 is a strong promoter taken from the BIOFAB collection. This device uses apFAB40 to express mRFP1, a red fluorescent protein. The apFAB40 test device consists of apFAB40, an RBS, the coding sequence for mRFP1, and a terminator.

This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.

The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.

iGEM: BBa_M36303 BIOFAB: apFAB46 Strength: 897897

iGEM: BBa_K2832101 BIOFAB: apFAB70 Strength: 866.7866.7

iGEM: BBa_K2832102 BIOFAB: apFAB71 Strength: 866866

iGEM: BBa_K2832103 BIOFAB: apFAB61 Strength: 857857

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 614
  Illegal AgeI site found at 726
  
• 1000
  COMPATIBLE WITH RFC[1000]