Coding

Part:BBa_K175004:Design

Designed by: Calin Plesa   Group: iGEM09_TUDelft   (2009-07-07)
Revision as of 15:02, 7 July 2009 by Cplesa (Talk | contribs) (→‎Design Notes)

I-PpoI homing endonuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 166


Design Notes

The Glutamine codon at 172 was changed from cag to caa in order to remove the PstI cut site.

Source

From slime mold (Physarum polycephalum), http://www.ncbi.nlm.nih.gov/protein/102295?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum

References