Composite

Part:BBa_K3790237

Designed by: Chongwen Cao   Group: iGEM21_Fudan   (2021-10-18)
Revision as of 23:14, 21 October 2021 by Chongwen (Talk | contribs)


GFP controlled by pBAD/araC


Introduction

2021 Fudan

the GFP is driven by pBAD promoter

the inhibitor of pBAD promoter araC is driven by pC



Usage and Biology

It can be used to generate GFP in E.coli. when induced by arabinose.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1885
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None