Regulatory

Part:BBa_K3726084

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-08)
Revision as of 23:09, 21 October 2021 by Sbarraga (Talk | contribs)

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P_PS-PR

This part is a strong constitutive promoter for cyanobacteria. The promoter sequence is composed by the fusion of a truncated variant of the rRNA promoter PR from Synechococcus PCC7942 and the PS promoter from E. coli.

Is one of the strongest promoters known in cyanobacteria, able to drive expression levels up to 15% of total protein.


Contribution from MADRID_UCM

Group: MADRID_UCM (4C_Fuels Project) Author: Jorge Fernández, Alvaro Luego, Santiago Barragán Ariza, Lucia Tapia Peña. Summary: Promoter PS-PR have demonstrated one of the highest expression levels in Synechococcus elongatus genre as well as related cyanobacteria species. This promoter element has been adapted to Phytobricks standard and succesfully assembled.

This phytobrick compatible promoter is part of a wider collection of regulatory elements for cyanobacteria, which are part of MADRID_UCM 2021 team contribution to iGEM community.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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