Intermediate

Part:BBa_K3725050

Designed by: Andrew Bae, Pranav Kanthala, Monica Cho, Janet Standeven, Megan McSweeney   Group: iGEM21_Lambert_GA   (2021-09-28)
Revision as of 22:45, 21 October 2021 by Pranavkanthala (Talk | contribs)


Phytophthora Toehold Switch Sequence

The Phytophthora Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. In the presence of the P. Cryptogea X24 gene, the hairpin loop will unfold and express the downstream GFP reporter protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None