Part:BBa_K3736001

constitutive promoter+ FourU + luxR + Terminator*2

constitutive promoter+ FourU + luxR + Terminator*2

Figure 1. Quorum sensing sequence

Gene Construct of DenTeeth

  We incorporate the whole part into E. coli BL21(DE3). We did colony PCR and digest to check its genotype.

Figure 2. Quorum sensing sequence + Restoration sequence + Sterilization sequence. M :1kb DNA ladder, 1 : Constitutive promoter + RBS + LuxR + Term. +Term. + Ptet + RBS + BMP2 + RBS + STATH + RBS + GFP + Term. + Term. + Plux + RBS + LL-37 + RBS + mRFP + RBS + tetR + Term. + Term. (4685 b.p.)</i>

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 890
  Illegal NgoMIV site found at 1011
  
• 1000
  COMPATIBLE WITH RFC[1000]