Signalling
Part:BBa_K4044009
Designed by: Kalinichenko Andrei Group: iGEM21_LMSU (2021-10-21)
BphP1-BcLOV4_QPAS1-Gal4 optogenetic system
there are several states of the ontogenetic system. Under the influence of far red light, BphP1 part of the chimeric protein BphP1-BcLOV4 becomes active and binds to Gal4-QPAS 1. This leads to the expression of target gene. Under the influence of red light, BphP1 is inactivated, Gal4-QPAS1 remains in the dimerized state. This leads to inhibition of transcription. At the same time, there may be residual activity of BphP1 and expression of target genes, which we do not need, therefore, under the influence of blue light, the entire BphP1-bclov4 complex binds to the lipid membrane, which completely eliminates the possibility of undesirable expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 815
Illegal PstI site found at 1112
Illegal PstI site found at 1559
Illegal PstI site found at 2608
Illegal PstI site found at 2852 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 815
Illegal PstI site found at 1112
Illegal PstI site found at 1559
Illegal PstI site found at 2608
Illegal PstI site found at 2852 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 301
Illegal BamHI site found at 1876
Illegal BamHI site found at 4029 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 815
Illegal PstI site found at 1112
Illegal PstI site found at 1559
Illegal PstI site found at 2608
Illegal PstI site found at 2852 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 815
Illegal PstI site found at 1112
Illegal PstI site found at 1559
Illegal PstI site found at 2608
Illegal PstI site found at 2852
Illegal NgoMIV site found at 457
Illegal NgoMIV site found at 1208
Illegal NgoMIV site found at 1705 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |