Coding

Part:BBa_K3783008:Experience

Designed by: Satvik Kethireddy   Group: iGEM21_OhioState   (2021-10-21)
Revision as of 22:30, 21 October 2021 by Satvik k (Talk | contribs) (→‎Applications of BBa_K3783008)

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Applications of BBa_K3783008

OhioState Application

LAL Assay Graph
Figure 2. Endotoxin Units of Construct Lysates via LAL Assay

The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a slight increase in endotoxin activity in the pFraB-LF. However, when looking at the hybrid promoter the endotoxin activity was less than the control

User Reviews

UNIQf96f89ce3b350f98-partinfo-00000001-QINU UNIQf96f89ce3b350f98-partinfo-00000002-QINU