Composite

Part:BBa_K4059027:Design

Designed by: Ann-Sofie Nielsen   Group: iGEM21_SDU-Denmark   (2021-10-21)
Revision as of 22:30, 21 October 2021 by AnnSofie1 (Talk | contribs) (→‎Design Notes)


CPR from Phanerochaete chrysosporium with N-terminal substitution (7), E. coli K12 codon optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 224
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One of the chimera N-terminals which gave the best expression level from the referenced study

Source

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References