Composite

Part:BBa_K3739111

Designed by: Shichen Geng   Group: iGEM21_XMU-China   (2021-09-30)
Revision as of 22:04, 21 October 2021 by Abby2333 (Talk | contribs) (→‎1. Agarose Gel Electrophoresis)


K525998(T7-RBS)-Aly01-PPO-his-B0010

Aly01 is a signal peptide from the alginate lyase of V.natriegens and PPO, a tyrosinase able to oxidize polyphenol, is to weaken the mfp glue.his-tag is used to be detected by anti-his antibody and purify the his-linking fusion

Biology

Aly01 is a signal peptide from the alginate lyase of V.natriegens. PPO, a tyrosinase able to oxidize polyphenol, is to weaken the mussel foot protein glue. his-tag is used to be detected by anti-his antibody and purify the his-linking fusion.

Usage

By codon optimization, the sequence suitable for expression in Vibrio Natriegens was constructed. Adding T7-RBS and terminator,construct new composite part BBa_K3739111.We transformed the constructed plasmid into Vibrio Natriegens to verify its successful heterologous expression. T--XMU-China--K3739111.png

Characterization

1. Agarose Gel Electrophoresis

After transferring the plasmid into Vibrio natriegens, colony PCR was used to certify the plasmid was correct. The expected result was obtained. T--XMU-China--K3739111.png

Reference

1. Holten-Andersen, N.; Waite, J. H., Mussel-designed protective coatings for compliant substrates. J Dent Res 2008, 87 (8), 701-9.

2. Kim, E.; Dai, B.; Qiao, J. B.; Li, W.; Fortner, J. D.; Zhang, F., Microbially Synthesized Repeats of Mussel Foot Protein Display Enhanced Underwater Adhesion. ACS Appl Mater Interfaces 2018, 10 (49), 43003-43012.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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