Composite

Part:BBa_K4059022:Design

Designed by: Ann-Sofie Nielsen   Group: iGEM21_SDU-Denmark   (2021-10-21)
Revision as of 22:04, 21 October 2021 by AnnSofie1 (Talk | contribs) (→‎Design Notes)


CPR from Phanerochaete chrysosporium with N-terminal substitution (2), E. coli K12 codon optimized


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 227
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One of the chimera N-terminals which gave the best expression level from the referenced study

Source

MALLLAVF source: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835717/


Aromatic rings in the transmembrane regions: https://www.nature.com/articles/s41598-019-48541-2

References