Part:BBa_K3971005

spp - Sucrose-Phosphate Phosphatase gene from Synechocystis PCC 6803

Usage and Biology

spp codes for Sucrose-phosphate phosphatase (SPP), an enzyme involved in the sucrose biosynthesis pathway in the cyanobacteria Synechocystis PCC 6803[1].

Fructose-6-phosphate is converted into sucrose-6-phosphate by the action of sucrose-phosphate synthase (SPS). Sucrose-phosphate phosphatase (SPP) then catalyses the conversion of sucrose-6-phosphate into sucrose [2].

In Synechocystis PCC 6803, SPS and SPP are coded for by two separate genes, sps, and spp [1].

In S. elongatus, SPS and SPP are natively found as a fused protein, and this fusion protein is coded for by a single sps gene[3].

Metabolic pathway of sucrose biosynthesis in Synechococcus elongatus UTEX 2973. Figure adapted from: Lin, Po-Cheng, Fuzhong Zhang, and Himadri B. Pakrasi. "Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973." Scientific reports 10.1 (2020): 1-8.

References

[1] Du, Wei, et al. "Exploring the photosynthetic production capacity of sucrose by cyanobacteria." Metabolic engineering 19 (2013): 17-25. https://doi.org/10.1016/j.ymben.2013.05.001 [2] Lin, Po-Cheng, Fuzhong Zhang, and Himadri B. Pakrasi. "Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973." Scientific reports 10.1 (2020): 1-8. [3] Duan, Y., Luo, Q., Liang, F. et al. Sucrose secreted by the engineered cyanobacterium and its fermentability. J. Ocean Univ. China 15, 890–896 (2016). https://doi.org/10.1007/s11802-016-3007-8

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]