Coding

Part:BBa_K4006004:Design

Designed by: Margaret Cook   Group: iGEM21_ASU   (2021-10-20)
Revision as of 21:43, 21 October 2021 by Mkcook (Talk | contribs) (→‎Design Notes)

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MT-6XHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 45
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5
    Illegal SapI.rc site found at 233


Design Notes

This part was designed for Golden Gate assembly and use with the type IIS restriction enzyme SapI in the compatible plasmid backbone pASapI. It is codon optimized for use in the chloroplast of Chlamydomonas reinhardtii. The 6X-His tag was added to the N terminal of the protein coding sequence to ensure there was no interaction with the functional domain of the folded protein.

Source

Part BBa_K3275000 - Human MT sequence

References