Coding

Part:BBa_K175000

Designed by: Calin Plesa   Group: iGEM09_TUDelft   (2009-07-05)
Revision as of 17:59, 5 July 2009 by Registry (Talk | contribs)

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trbC (pilin) of IncP beta R751 plasmid

The relaxase / helicase TraI of the IncP beta plasmid R751. Essential of conjugative transfer. This part nicks oriT. It should be placed on a message plasmid along with oriT, and will enable transfer of the message plasmid in the presence of a modified R751 helper plasmid such as BBa_I714037.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 217
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//dna/conjugation
//function/conjugation
Parameters
directionForward
n/atrbC (pilin) of IncP beta R751 plasmid