Part:BBa_K4088892

DnaE - N

N-terminal of Npu DnaE intein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 260
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Usage and Biology

Intein
Intein is a segment of a protein that can self-catalytically excised out and ligate the remaining parts of the protein (N- and C-exteins) with a peptide bond ^[1].
For this part we used the Npu DnaE intein. This intein is identified as a naturally occurring split mini-intein in Synechocystis sp. PCC6803 ^[2]. Its first amino acid is cysteine which is important for protein trans-splicing to take place.

This part we used in the сomposite system - N-terminal fragment of β-lactamase fused with dCas13a and N-terminal intein (BBa_K4088891).

References

↑ Anraku, Y., Mizutani, R. and Satow, Y. (2005), Protein Splicing: Its Discovery and Structural Insight into Novel Chemical Mechanisms. IUBMB Life, 57: 563-574. https://doi.org/10.1080/15216540500215499
↑ Wu, H., Hu, Z., & Liu, X. Q. (1998). Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. Proceedings of the National Academy of Sciences of the United States of America, 95(16), 9226–9231. https://doi.org/10.1073/pnas.95.16.9226

[1] Anraku, Y., Mizutani, R. and Satow, Y. (2005), Protein Splicing: Its Discovery and Structural Insight into Novel Chemical Mechanisms. IUBMB Life, 57: 563-574. https://doi.org/10.1080/15216540500215499

[2] Wu, H., Hu, Z., & Liu, X. Q. (1998). Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. Proceedings of the National Academy of Sciences of the United States of America, 95(16), 9226–9231. https://doi.org/10.1073/pnas.95.16.9226

[1]

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