Coding

Part:BBa_K3735888

Designed by: Guolong Shi   Group: iGEM21_NAU-CHINA   (2021-10-21)
Revision as of 21:03, 21 October 2021 by Jiandong (Talk | contribs)

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SeNPs production parts

In order to explore the optimal conditions for the production of SeNPs for BBa_K3735004, we used the orthogonal experimental model-Response surface model (more details see CO Model).

We hope to maximize SeNPs production by optimizing temperature, pH and selenite concentration. First, we conducted a preliminary experiment on these three factors, and we chose the best three gradients(Fig.1) of these three factors to do further experiments.

Fig.1 Preliminary experiment results of response surface experiment. A: pH; B: concentration of selenite; C: Temperature. The results of preliminary experiment show that the SeNPs yield is the best when pH are 6,7,8 temperature are 16°C,30°C,37°C and selenite concentration are 12mM,15mM,18mM.

Second, according to preliminary results and setting gradients to explore the interaction between three different types of conditions—pH 6, 7, and 8, temperature 16℃, 30℃, and 37℃, selenite concentration 12, 15, and 18mM, and conducted experiments as shown in the Fig.2.

Fig.2 Response surface experiment of SeNPs synthesis.

We used response surface model to simulate the optimal SeNPs production conditions (more details see CO Model)). Finally, we obtained the optimal conditions and maximum yield as follows:
Solutions:

By response surface model analysis, we simulated the optimal experimental conditions of SeNPs production: temperature at 30.27℃, selenite concentration at 14.33mM, pH at 6.75. Finally, the optimal SeNPs yield of 0.0315562g was obtained.

Fig.3 the final product of our project.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
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  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1537
    Illegal BglII site found at 2854
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  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
    Illegal NgoMIV site found at 644
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 1151
    Illegal NgoMIV site found at 1574
    Illegal NgoMIV site found at 2675
    Illegal NgoMIV site found at 2924
    Illegal AgeI site found at 671
    Illegal AgeI site found at 1217
    Illegal AgeI site found at 1373
    Illegal AgeI site found at 1514
    Illegal AgeI site found at 1937
    Illegal AgeI site found at 2294
    Illegal AgeI site found at 2465
  • 1000
    COMPATIBLE WITH RFC[1000]


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