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Coding
davB
Part:BBa_K3776001
Designed by: Helene Heling Huang Group: iGEM21_HK_SSC (2021-10-01)
Revision as of 20:45, 21 October 2021 by Meowstarperson (Talk | contribs)
DavB
DavB, L-Lysine monooxygenase, catalyzes the reaction of L-lysine to 5-aminovaleramide in the natural 5-aminovalerate pathway in Pseudomonas putida KT2440. This DavB sequence has been codon optimized for expression in Synechococcus elongatus UTEX 2973.
References
Liu, P., Zhang, H., Lv, M., Hu, M., Li, Z., Gao, C., Xu, P., & Ma, C. (2014). Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase. Scientific Reports, 4(1). https://doi.org/10.1038/srep05657
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 239
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1328
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1488
Illegal AgeI site found at 895
Illegal AgeI site found at 1144 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |