Composite

Part:BBa_K4059014:Design

Designed by: Ann-Sofie Nielsen   Group: iGEM21_SDU-Denmark   (2021-10-21)
Revision as of 20:41, 21 October 2021 by AnnSofie1 (Talk | contribs) (→‎Design Notes)


PsiH from Psilocybe cubensis with N-terminal substitution (4), E. coli K12 codon optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 833
    Illegal SapI.rc site found at 455


Design Notes

One of the chimera N-terminals which gave the best expression level from the referenced study

Source

,

References