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Part:BBa_K3736000
STATH(Statherin)
A protein that promotes tooth remineralization and repairs decalcified enamel.
Statherin is a protein in humans that is encoded by the STATH gene. It prevents the precipitation of calcium phosphate in saliva, maintaining a high calcium level in saliva available for remineralization of tooth enamel and high phosphate levels for buffering.
STATH Functional Test
Carbon rods are processed to have small, low-volume pores that increase the surface area available for adsorption. We expected that both STATH and calcium could adsorb on the surface of carbon rods, so we designed a test to observe the surface of carbon rods soaked in different solutions under the microscope.
We made four solutions configured to calcium ions saturated solutions by the calcium chloride solution, ddH2O, LB broth, DenTeeth, and E. coli with pSB1K3, in the tubes with the carbon rods stuck inside. After putting them into a 0°C refrigerator for 12 hours, the solutions would become calcium ions supersaturated solutions. In the DenTeeth, STATH would bind onto the surface of the carbon rod, preventing the calcium ions from binding onto it.
As for the precipitation on the bottom of tubes was the debris of bacteria, which was died of the broken cell membrane caused by the high concentration of calcium chloride solution.
The result in the following figure showed that the surface of the carbon rod taken out of the DenTeeth was matte compared with that in other solutions. We presumed that the result was due to the protective layer on the surface formed by the STATH that would prevent the liquid from attachment.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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